Journal: Nature Communications

Article Title: Ybx1 fine-tunes PRC2 activities to control embryonic brain development

doi: 10.1038/s41467-020-17878-y

Figure Lengend Snippet: WB analysis of IgG, JARID2, and YBX1 co-IP of a the hESC nuclear extract with high-stringency washes and b the hNPC nuclear extract. c WB analysis of IgG and SUZ12 co-IP of the hNPC nuclear extract. d WB analysis of IgG, Jarid2, and Ybx1 co-IP of the mouse neural tube nuclear extract. WB analysis of His-JARID2 and e GST-YBX1 or f myc-tagged YBX1 protein fragments. Source data are provided in file.

Article Snippet: We confirmed the JARID2–YBX1 binding with new IP-mass spectrometry datasets using a different JARID2 antibody (R&D Systems AF6090) in mouse neural stem cells NE-4C (Supplementary Fig. ).

Techniques: Co-Immunoprecipitation Assay

Journal: Nature Communications

Article Title: Ybx1 fine-tunes PRC2 activities to control embryonic brain development

doi: 10.1038/s41467-020-17878-y

Figure Lengend Snippet: WB analysis of IgG, JARID2, and YBX1 co-IP of a the hESC nuclear extract with high-stringency washes and b the hNPC nuclear extract. c WB analysis of IgG and SUZ12 co-IP of the hNPC nuclear extract. d WB analysis of IgG, Jarid2, and Ybx1 co-IP of the mouse neural tube nuclear extract. WB analysis of His-JARID2 and e GST-YBX1 or f myc-tagged YBX1 protein fragments. Source data are provided in file.

Article Snippet: Full-length recombinant protein was bound overnight at 4 °C to PierceTM Protein A/G Magnetic Beads (ThermoFisher Scientific) with JARID2 antibody (Novus Biologicals).

Techniques: Co-Immunoprecipitation Assay